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cd25 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd25 antibody, anti-human, reafinity
    Cd25 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25/CD25+Antibody%2C+anti-human%2C+REAfinity/custom%40130-122-302%4042526444
    Average 93 stars, based on 76 article reviews
    cd25 antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Lipoic acid improves wound healing through its immunomodulatory and anti-inflammatory effects in a diabetic mouse model.
    Article Snippet: 1 Cell Biology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt 2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Salman International University, Ras Sudr, South Sinai, Egypt 3 Hormones Department, Medical Research and Clinical Studies Institute, and Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research Centre, Dokki, Cairo 12622, Egypt 4 Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O.. BOX 84428, 11671 Riyadh, Saudi Arabia 5 Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia 6 Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt Abstract Objectives Diabetes mellitus is a chronic disease that has become more prevalent worldwide because of lifestyle changes.. It leads to serious complications, including increased atherosclerosis, protein glycosylation, endothelial dysfunction, and vascular denervation.

    Bioprocessing:

    Article Title: Lipoic acid improves wound healing through its immunomodulatory and anti-inflammatory effects in a diabetic mouse model.
    Article Snippet: 1 Cell Biology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt 2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Salman International University, Ras Sudr, South Sinai, Egypt 3 Hormones Department, Medical Research and Clinical Studies Institute, and Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research Centre, Dokki, Cairo 12622, Egypt 4 Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O.. BOX 84428, 11671 Riyadh, Saudi Arabia 5 Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia 6 Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt Abstract Objectives Diabetes mellitus is a chronic disease that has become more prevalent worldwide because of lifestyle changes.. It leads to serious complications, including increased atherosclerosis, protein glycosylation, endothelial dysfunction, and vascular denervation.

    Article Title: Systemic miR-26a deficiency attenuates pulmonary fibrosis via PTEN upregulation and downstream TIMP-1 suppression
    Article Snippet: .. Cells were treated with TruStain FcX (93, BioLegend) followed by staining with fluorochrome-conjugated antibodies or biotinylated monoclonal antibodies CD19 (clone 6D5), B220 (clone RA3-6B2), IgD (clone 11-26c.2a), IgM (clone RMM-1), CD5 (clone 53–7.3), CD21 (clone 7E9), CD38 (clone 90), CD73 (clone TY/11.8), CD138 (clone 281–2), and TACI (clone 8F10) from BioLegend; CD23 (clone B3B4) from Invitrogen; CD25 (clone REAL537) from Miltenyi Biotec; and AA4.1 (clone 493), Fas (clone SA367H8), CD4 (clone RM4-5), CD8 (clone 53–6.7), CD44 (clone IM7), and CD62L (clone MEL-14) from BD Biosciences. .. APC-conjugated streptavidin (BD Biosciences) and BV650-conjugated streptavidin (BioLegend) were used to detect the biotin-marked cells.

    Article Title: Systemic miR-26a deficiency attenuates pulmonary fibrosis via PTEN upregulation and downstream TIMP-1 suppression
    Article Snippet: .. Cells were treated with TruStain FcX (93, BioLegend) followed by staining with fluorochrome-conjugated antibodies or biotinylated monoclonal antibodies CD19 (clone 6D5), B220 (clone RA3-6B2), IgD (clone 11-26c.2a), IgM (clone RMM-1), CD5 (clone 53–7.3), CD21 (clone 7E9), CD38 (clone 90), CD73 (clone TY/11.8), CD138 (clone 281–2), and TACI (clone 8F10) from BioLegend; CD23 (clone B3B4) from Invitrogen; CD25 (clone REAL537) from Miltenyi Biotec; and AA4.1 (clone 493), Fas (clone SA367H8), CD4 (clone RM4-5), CD8 (clone 53–6.7), CD44 (clone IM7), and CD62L (clone MEL-14) from BD Biosciences. .. APC-conjugated streptavidin (BD Biosciences) and BV650-conjugated streptavidin (BioLegend) were used to detect the biotin-marked cells.

    Blocking Assay:

    Article Title: Lipoic acid improves wound healing through its immunomodulatory and anti-inflammatory effects in a diabetic mouse model.
    Article Snippet: 1 Cell Biology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt 2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Salman International University, Ras Sudr, South Sinai, Egypt 3 Hormones Department, Medical Research and Clinical Studies Institute, and Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research Centre, Dokki, Cairo 12622, Egypt 4 Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O.. BOX 84428, 11671 Riyadh, Saudi Arabia 5 Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia 6 Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt Abstract Objectives Diabetes mellitus is a chronic disease that has become more prevalent worldwide because of lifestyle changes.. It leads to serious complications, including increased atherosclerosis, protein glycosylation, endothelial dysfunction, and vascular denervation.

    Staining:

    Article Title: Systemic miR-26a deficiency attenuates pulmonary fibrosis via PTEN upregulation and downstream TIMP-1 suppression
    Article Snippet: .. Cells were treated with TruStain FcX (93, BioLegend) followed by staining with fluorochrome-conjugated antibodies or biotinylated monoclonal antibodies CD19 (clone 6D5), B220 (clone RA3-6B2), IgD (clone 11-26c.2a), IgM (clone RMM-1), CD5 (clone 53–7.3), CD21 (clone 7E9), CD38 (clone 90), CD73 (clone TY/11.8), CD138 (clone 281–2), and TACI (clone 8F10) from BioLegend; CD23 (clone B3B4) from Invitrogen; CD25 (clone REAL537) from Miltenyi Biotec; and AA4.1 (clone 493), Fas (clone SA367H8), CD4 (clone RM4-5), CD8 (clone 53–6.7), CD44 (clone IM7), and CD62L (clone MEL-14) from BD Biosciences. .. APC-conjugated streptavidin (BD Biosciences) and BV650-conjugated streptavidin (BioLegend) were used to detect the biotin-marked cells.

    Article Title: A novel Vδ1 engager targeting CD19 enhances human Vδ1 γδ T cell responses against CLL and CD19+ hematological malignancies.
    Article Snippet: γδ T cells are associated with favorable outcomes in many cancers likely through a mechanism of stress-directed cytotoxicity and antitumor cytokine production.. Vδ1 γδ T cells are especially promising for immunotherapy due to their broad stress recognition and resistance to activation-induced cell death.. Here, we generate a CD19 engager incorporating a novel Vδ1 binding moiety as proof-of-concept for treating CD19 cancers, including Chronic Lymphocytic Leukemia (CLL).

    Article Title: Systemic miR-26a deficiency attenuates pulmonary fibrosis via PTEN upregulation and downstream TIMP-1 suppression
    Article Snippet: .. Cells were treated with TruStain FcX (93, BioLegend) followed by staining with fluorochrome-conjugated antibodies or biotinylated monoclonal antibodies CD19 (clone 6D5), B220 (clone RA3-6B2), IgD (clone 11-26c.2a), IgM (clone RMM-1), CD5 (clone 53–7.3), CD21 (clone 7E9), CD38 (clone 90), CD73 (clone TY/11.8), CD138 (clone 281–2), and TACI (clone 8F10) from BioLegend; CD23 (clone B3B4) from Invitrogen; CD25 (clone REAL537) from Miltenyi Biotec; and AA4.1 (clone 493), Fas (clone SA367H8), CD4 (clone RM4-5), CD8 (clone 53–6.7), CD44 (clone IM7), and CD62L (clone MEL-14) from BD Biosciences. .. APC-conjugated streptavidin (BD Biosciences) and BV650-conjugated streptavidin (BioLegend) were used to detect the biotin-marked cells.

    Article Title: Multispecific anti-TCR delta variable 1 antibodies
    Article Snippet: Skin γδ T-cells (from donor ATS006) were seeded into a 96 well round bottom plate at 6×10{circumflex over ( )}5 cells/ml in γδ media with THP-1 cells (ATCC®-TIB-202) at 1.2×10{circumflex over ( )}6 cells/ml and increasing concentration of the test mAbs (range from 0.00067 to 67 nM) or the highest concentration (67 nM) of the corresponding isotype control (higG1, RSV), diluted in PBS. .. The cells were incubated for 24 hours in a humidified CO2 chamber at 37° C. The cells were washed and stained for dead cells (THERMO FISHERTM) #15580607), VD1 TCRs (MILTENYI® #130-117-697) and CD25 (MILTENYI® #130-113-280) for 30 minutes at 4° C. The cells were washed in FACS buffer before incubating overnight at 4° C. in the dark. ..

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells
    Article Snippet: .. 1x10 5 cells per condition were used for cell surface marker staining for CXCR4 (APC, Miltenyi Biotec, #130-120-708 [clone REA649]), CD4 (PE, Miltenyi Biotec, #130-113-225 [clone REA623]), CD25 (APC, Miltenyi Biotec, #130-115-535 [clone REA945]), and CCR5 (APC, Miltenyi Biotec, #130-120-708 [clone REA245]) at day 5 post-editing. ..

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells.
    Article Snippet: .. 1x105 cells per condition were used for cell surface marker staining for CXCR4 (APC, Miltenyi Biotec, #130-120-708 [clone REA649]), CD4 (PE, Miltenyi Biotec, #130-113-225 [clone REA623]), CD25 (APC, Miltenyi Biotec, #130-115-535 [clone REA945]), and CCR5 (APC, Miltenyi Biotec, #130-120-708 [clone REA245]) at day 5 post-editing. ..

    Flow Cytometry:

    Article Title: A novel Vδ1 engager targeting CD19 enhances human Vδ1 γδ T cell responses against CLL and CD19+ hematological malignancies.
    Article Snippet: γδ T cells are associated with favorable outcomes in many cancers likely through a mechanism of stress-directed cytotoxicity and antitumor cytokine production.. Vδ1 γδ T cells are especially promising for immunotherapy due to their broad stress recognition and resistance to activation-induced cell death.. Here, we generate a CD19 engager incorporating a novel Vδ1 binding moiety as proof-of-concept for treating CD19 cancers, including Chronic Lymphocytic Leukemia (CLL).

    Incubation:

    Article Title: Multispecific anti-TCR delta variable 1 antibodies
    Article Snippet: Skin γδ T-cells (from donor ATS006) were seeded into a 96 well round bottom plate at 6×10{circumflex over ( )}5 cells/ml in γδ media with THP-1 cells (ATCC®-TIB-202) at 1.2×10{circumflex over ( )}6 cells/ml and increasing concentration of the test mAbs (range from 0.00067 to 67 nM) or the highest concentration (67 nM) of the corresponding isotype control (higG1, RSV), diluted in PBS. .. The cells were incubated for 24 hours in a humidified CO2 chamber at 37° C. The cells were washed and stained for dead cells (THERMO FISHERTM) #15580607), VD1 TCRs (MILTENYI® #130-117-697) and CD25 (MILTENYI® #130-113-280) for 30 minutes at 4° C. The cells were washed in FACS buffer before incubating overnight at 4° C. in the dark. ..

    FACS:

    Article Title: Multispecific anti-TCR delta variable 1 antibodies
    Article Snippet: Skin γδ T-cells (from donor ATS006) were seeded into a 96 well round bottom plate at 6×10{circumflex over ( )}5 cells/ml in γδ media with THP-1 cells (ATCC®-TIB-202) at 1.2×10{circumflex over ( )}6 cells/ml and increasing concentration of the test mAbs (range from 0.00067 to 67 nM) or the highest concentration (67 nM) of the corresponding isotype control (higG1, RSV), diluted in PBS. .. The cells were incubated for 24 hours in a humidified CO2 chamber at 37° C. The cells were washed and stained for dead cells (THERMO FISHERTM) #15580607), VD1 TCRs (MILTENYI® #130-117-697) and CD25 (MILTENYI® #130-113-280) for 30 minutes at 4° C. The cells were washed in FACS buffer before incubating overnight at 4° C. in the dark. ..

    Isolation:

    Article Title: Teriflunomide Inhibits Human FOXP3 + Regulatory T Cell Function by Interference With Mitochondrial Respiration.
    Article Snippet: In brief, PBMCswere isolated by Ficoll-Paque PLUS (GE17-1440-02, Sigma) gradient centrifugation. .. CD25−-enriched and CD25−-depleted PBMCs were isolated with CD25 microbeads II (130-097-044, Miltenyi Biotec). ..

    Marker:

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells
    Article Snippet: .. 1x10 5 cells per condition were used for cell surface marker staining for CXCR4 (APC, Miltenyi Biotec, #130-120-708 [clone REA649]), CD4 (PE, Miltenyi Biotec, #130-113-225 [clone REA623]), CD25 (APC, Miltenyi Biotec, #130-115-535 [clone REA945]), and CCR5 (APC, Miltenyi Biotec, #130-120-708 [clone REA245]) at day 5 post-editing. ..

    Article Title: Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cells.
    Article Snippet: .. 1x105 cells per condition were used for cell surface marker staining for CXCR4 (APC, Miltenyi Biotec, #130-120-708 [clone REA649]), CD4 (PE, Miltenyi Biotec, #130-113-225 [clone REA623]), CD25 (APC, Miltenyi Biotec, #130-115-535 [clone REA945]), and CCR5 (APC, Miltenyi Biotec, #130-120-708 [clone REA245]) at day 5 post-editing. ..



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    a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
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    a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
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    a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
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    a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs <t>(CD4+CD25+)</t> of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .
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    Image Search Results


    a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs (CD4+CD25+) of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .

    Journal: Nature Communications

    Article Title: Nrf2-mediated metabolic reprogramming drives regulatory T cell accumulation in hepatocellular carcinoma

    doi: 10.1038/s41467-026-73485-3

    Figure Lengend Snippet: a Representative gating strategy of Nrf2-activated cells (HO-1+) within Tregs (CD127 lo CD25 hi FoxP3+) and CD4+ Tconv (CD127+ CD25−). b , c Frequency of live and Annexin V+ cells within HO-1+ or HO-1-Tregs and CD4+ Tconv. d – i Frequency of live and Annexin V+ cells in donor Tregs (CD4+CD127 lo CD25 hi ) and Tconv (CD4+ CD25− and CD8+) before (baseline) and after 24 h culture in RPMI standard or high lactate and low glucose with and without the NRF2 inhibitor ML385. j , k Frequency of live and Annexin V+ cells of Tregs (CD4+ CD25+) and Teff (CD4+ CD25− and CD8+) of WT and Nrf2 −/− mice after 24 h culture in RPMI standard or high lactate and low glucose. l Example histograms of the mean fluorescence intensity (MFI) of MtGreen and MtDR in freshly isolated Tregs (CD4+CD25+) of WT and Nrf2 −/− mice. m Ratio of the geometric MFI (gMFI) of MtGreen and MtDR and n radiometric index on freshly isolated Tregs, Tconv (CD4+ CD25− or CD8+) of WT and Nrf2 −/− mice. The radiometric index was calculated as the difference between the log₁₀-transformed MFI of MtDR and the log₁₀-transformed MFI of MtGreen. o Example histogram on Tregs and CD4+ Tconv (left, CD4+CD25+ and CD4+ CD25−) and mean MFI (right) of MtGreen and MtDR in HCC samples. Data are presented as mean values + SD or ±SD as appropriate. P -values were calculated by two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test for ( b – n ); or paired t-test (two-sided) for ( o ). Only statistically significant P -values are displayed: *, P < 0.05; **, P < 0.01; ***, P < 0.001. MtDR, mitotracker deep red; MtGreen, mitotracker green. FACS sequential gating strategy for ( d – k ) is provided in Supplementary Fig. .

    Article Snippet: Freshly isolated human healthy CD4+CD25+ Tregs were pre-incubated in the presence or absence of 100 nM of the MCT1 inhibitor AZD3965 (MedChemExpress; HY-12750) for 2 h and then cultured with low dose IL-2 (50IU/ml) and 1:5 aCD3/CD28 beads in RPMI standard or RPMI high lactate and low glucose (as described above) for 30 min.

    Techniques: Fluorescence, Isolation, Transformation Assay